← All apps PCR Amplification Simulator
Education
Run the thermal cycler and watch DNA double each cycle, tracked strand by strand so you can see when the product of exactly the right length first appears and then takes over. Add a realistic efficiency and a plateau, then switch to a real-time qPCR view and read Ct values from a dilution series, with the slope giving your efficiency. A primer checker reports amplicon length, GC content, melting temperature and common problems like primer dimers.
Open PCR Amplification Simulator (pcramplification.apps.vtc.com)
About PCR Amplification Simulator
PCR Amplification Simulator is an interactive learning tool on VTC. Run the thermal cycler and watch DNA double each cycle, tracked strand by strand so you can see when the product of exactly the right length first appears and then takes over. Add a realistic efficiency and a plateau, then switch to a real-time qPCR view and read Ct values from a dilution series, with the slope giving your efficiency. A primer checker reports amplicon length, GC content, melting temperature and common problems like primer dimers. It runs in your web browser and is free to use, and suits students, teachers and self-learners.
Who it's for: Students, teachers and self-learners
Features
- Add a realistic efficiency and a plateau, then switch to a real-time qPCR view and read Ct values from a dilution series
- The slope giving your efficiency
- A primer checker reports amplicon length, GC content, melting temperature and common problems like primer dimers
- Free to use in your browser
Frequently asked questions
What is PCR Amplification Simulator on VTC?
PCR Amplification Simulator is an interactive learning tool on VTC. Run the thermal cycler and watch DNA double each cycle, tracked strand by strand so you can see when the product of exactly the right length first appears and then takes over. Add a realistic efficiency and a plateau, then switch to a real-time qPCR view and read Ct values from a dilution series, with the slope giving your efficiency.
Is PCR Amplification Simulator free to use?
Yes. The VTC app catalog lists PCR Amplification Simulator at no cost, and it runs in your web browser with nothing to install.
Who is PCR Amplification Simulator for and how do I start?
It is aimed at students, teachers and self-learners. To start, open https://pcramplification.apps.vtc.com in your browser.